Amanita Research Forum

I'm so sorry for the late reply @constrapolator ! But YES!!!!!!! Congrats!!! Hopefully you transferred it? Exciting to hear! Yep, I transferred to a new sanitized container with the same ground barley material. The image on the left shows the original container (likely with contaminants), and the i...

Alright, it looks like the crushed caramel barley is the winner! After three days, I didn't see as much activity on the other media. Mcpato , does this look like fleece to you? PXL_20210710_185654821.jpg If so, I'll transplant it to another sterilized crushed barley jar to try to isolate/propagate it.

Here's what I did (all equipment used was sterilized): 1. Prepare 3 jars. One has wheat barley, one has crushed caramel barley, and one has uncrushed caramel barley. Fill with water and pasteurize for 2 hours at 190F. 2. Filter out the water in each jar, and add a few tablespoons back to the jar. 3....

@Mcpato Thanks so much for your guidance!

I'll give it a shot as described, and post lots of pictures shortly.

Mcpato About to give the "living bread" method a shot. Any guidance would be appreciated, as there's a lot of different things attempted in different threads, and I'm not sure what the best approach is. From my understanding, steps would be something like: That is: 1. Sterliize/pasteurize barley, s...