Amanita Research Forum

just checked the pH of the Lactobacillus brevis culture I did inoculate 2 jars with 30g/l sugar and a little sprinkle yeast nutrients, and in one of the two jars the pH was manipulated down to ~5 with tartraric acid, this is as i already mentioned my first time culuring an isolated bacteria strain (...

@fantanyl Thank you so much for simplifying your hypothesis for me! I am a little embarrassed that I am not nearly as fluent in the lingo, nor the chemistry you are clearly adept in! Thanks Mcpato ! It means a lot to me that my lingo is well recieved, because i think as you already recognized, engl...

So after the maybe a little confusion like post at some points yesterday i wanted to clear the mess up a little bit. This post is dedicated to the Structure similarities thing i talked about. So my claim is as already said, the GAD could have the same impact (decarboxylation) on the mystery compound...

So, after a little gearing up and doing some research im finally back with new plans :) , As already said Lostmushroomforests´s replys on my experiments got me major thinking. So before going on culturing the fleece on greater scale i definitly have to know more exactly what i actually have in cultu...

fantanyl If there is acidification happening, you may already have lactobacillus present in the brew. It looks like you have a vinegar or kombucha culture growing with the fleece mold - so instead of a SCOBY (symbiotic culture of bacteria and yeasts) you have a SCOMBY (symbiotic culture of mold, ba...

of course there are holes at the top of the jars, covererd with micropore tape, so airchange is possible but nothing else (but i think a piece of cloth or something like that would be also fine, because its not sterile inside anyway) mpt 1.jpg mpt2.jpg with this method its much easyer to fully colon...

sry i had to splitt the post, because i wasnt able to post the other pics (maybe datalimit per post ?) so here the fleece still floats haha floatin 2.jpg and after one, two and three days it has already colonised a big part of the surface of the grape juice: day 1.jpg day two.jpg day three.jpg day t...

Thanks Mcpato ! yeah giving up is absolutly no option, i´m at the very beginning at this moment :) I actually did a couple of trys with the raw ambrosia of my last batch, the effects seem to be more consistent than with the decarb process, but i´m sure i´m doing something wrong with the decarb, i´ll...

sooo, i did a couple of tests with my first batches of ambrosia, one with my first little batch only sat for 5 days or so. this was far too less time and tasted not really different then just the original grape juice (decarbed in double boiler and diluited) ~200 ml before diluition and felt nothing ...

thanks Mcpato ! really appreciate your experienced eye for the fleece and your opinion ! in the attachments is an update of today, i think now its a bit more unmistakeable the fleece, but i wasn´t really sure because its thin appearence, in all the pics you can find in the net on top of the ambrosia...