Fucomannogalactan and glucan from mushroom Amanita muscaria: Structure and inflammatory pain inhibition
Posted: Tue Oct 01, 2019 8:52 pm
https://core.ac.uk/download/pdf/8268935 ... d5xkSWbzWg
Abstract: A fucomannogalactan (FMG-Am) and a (1 → 3), (1 → 6)-linked -d-glucan (GLC-Am) were isolated from Amanita muscaria fruiting bodies. These compounds’ structures were determined using mono- and bi- dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. FMG-Am was shown to be a heterogalactan formed by a (1 → 6)-linked -d-galactopyranosyl main chain partially sub- stituted at O-2 mainly by -l-fucopyranose and a minor proportion of -d-mannopyranose non-reducing end units. GLC-Am was identified as a (1 → 3)-linked -d-glucan partially substituted at O-6 by mono- and a few oligosaccharide side chains, which was confirmed after controlled Smith degradation. Both the homo- and heteropolysaccharide were evaluated for their anti-inflammatory and antinociceptive potential, and they produced potent inhibition of inflammatory pain, specifically, 91 ± 8% (30 mg kg−1 ) and 88 ± 7% (10 mg kg−1 ), respectively.
Abstract: A fucomannogalactan (FMG-Am) and a (1 → 3), (1 → 6)-linked -d-glucan (GLC-Am) were isolated from Amanita muscaria fruiting bodies. These compounds’ structures were determined using mono- and bi- dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. FMG-Am was shown to be a heterogalactan formed by a (1 → 6)-linked -d-galactopyranosyl main chain partially sub- stituted at O-2 mainly by -l-fucopyranose and a minor proportion of -d-mannopyranose non-reducing end units. GLC-Am was identified as a (1 → 3)-linked -d-glucan partially substituted at O-6 by mono- and a few oligosaccharide side chains, which was confirmed after controlled Smith degradation. Both the homo- and heteropolysaccharide were evaluated for their anti-inflammatory and antinociceptive potential, and they produced potent inhibition of inflammatory pain, specifically, 91 ± 8% (30 mg kg−1 ) and 88 ± 7% (10 mg kg−1 ), respectively.